Journal: Journal of visualized experiments : JoVE

Article Title: Studying Normal Tissue Radiation Effects using Extracellular Matrix Hydrogels

doi: 10.3791/59304

Figure Lengend Snippet:

Article Snippet: Compound Fisher Scientific 23-730-571 cryostat embedding medium Fluoromount-G SouthernBiotech 0100–01 aqueous based mounting medium FreeZone 4.5 Labconco 7751020 lyophilizer Hoechst 33342 Solution (20 mM) Thermo Scientific 62249 blue fluorescent dye Hydrochloric acid Sigma-Aldrich 258148–500ML - IVIS Lumina III PerkinElmer CLS136334 bioluminescence imaging system Kimtech Science Kimwipes Kimberly Clark delicate task wipes n-Propanol (Peroxide-Free/Sequencing), Fisher BioReagents Fisher Scientific BP1130–500 - Oil Red O Sigma-Aldrich O0625–25G 1-([4-(Xylylazo)xylyl]azo)-2- naphthol OPS Diagnostics CryoGrinder OPS Diagnostics, LLC CG-08–02 - PBS (10X), pH 7.4 Quality Biological, Inc. 119-069-151 Phosphate-buffered saline Penicillin-Streptomycin Gibco 15140–122 - Pepsin from porcine gastric mucosa Sigma-Aldrich P6887–5G pepsin Peracetic acid Sigma-Aldrich 77240–100ML - Phalloidin-iFluor 594 Reagent (ab176757) abcam ab176757 phalloidin conjugate Propylene glycol Sigma-Aldrich W294004–1KG-K - Richard-Allan Scientific Signature Series Bluing Reagent Richard-Allan Scientific 7301L bluing agent Richard-Allan Scientific Signature Series Hematoxylin 7211 Richard-Allan Scientific 7211 - RPMI Medium 1640 Gibco 11875–093 - Sodium deoxycholate, 98% Frontier Scientific JK559522 deoxycholic acid Sucrose Sigma-Aldrich S5016 - Triton x-100 Sigma-Aldrich X100–100ML t -Octylphenoxypolyethoxyethanol Trypsin-EDTA (0.25%), phenol red Gibco 25200–056 - Whatman qualitative filter paper, Grade 4 Whatman 1004–110 grade 4 qualitative filter paper Xylenes (Certified ACS), Fisher Chemical Fisher Scientific X5–4 - Open in a separate window

Techniques: Imaging